Fig. 7

Plumbagin retards the WTAP-mediated granulosa cell pyroptosis. Overexpress WTAP in GC cell line HGL5 by transfecting with a recombinant plasmid containing WTAP. The cells were then treated with plumbagin. A Cell survival imaged by LIVE/DEAD™ Cell Imaging kit. B LDH production by dead cells was quantified by LDH kits. N = 4. C Cell viability was tested with alamarBlue staining. n = 4. D FLICA probe stain for activated Caspase-1 (Representative graph). E FLICA probe stain for activated Caspase-1(statistic).n = 4. F IL-1β production by HGL-5 cells were quantified by ELISA. N = 4. G IL-18 production by HGL 5 cells were determined by ELISA. N = 4. Individual data points are displayed. Data are mean ± SEM. Paired one-way ANOVA was used to analyze the difference. *P < 0.05; **P < 0.01; ***P < 0.001