Fig. 2

ANGPTL4 inhibition attenuated OC metastasis. A Representative image showing cell migration in the monolayer wound healing assay. Images were obtained at 0 and 24 h after standard wounding. Scale bars, 100 μm. B Cell migration assays were performed using 24-well Transwell plates at 24 h after plating. Scale bars, 400 μm. C Cell invasion assays were performed using 24-well Transwell plates coated with Matrigel at 24 h after plating. The data are from at least three independent experiments and are shown as the means ± SD. One-way ANOVA. D Representative pictures of peritoneal metastasis in a xenograft tumor model generated by the intraperitoneal injection of LV-shCon SKOV3 cells and LV-shANGPTL4 SKOV3 cells (n = 8 in each group). E Box plot of the weight of metastatic tumor nodules in the abdominal cavities of the groups injected with the LV-shCon SKOV3 cell and LV-shANGPTL4 SKOV3 cell lines. F The quantification bar graph shows all implanted nodule volumes in the abdominal cavity of nude mice. E Gross morphology of tumor sizes. Scale bars, 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001; NS indicates not significant