Fig. 4

ANGPTL4 promotes OC angiogenesis in vitro. A LV-shANGPTL4 cell-derived CM inhibited HUVEC tube formation compared with that in the LV-Con groups, and 250 ng/ml rhANGPTL4 treatment increased HUVEC tube formation compared with that in the PBS group, as shown by tube formation assays. Scale bars, 100 μm. B LV-shANGPTL4-cell-derived CM decreased the proliferation of HUVECs compared to that in the LV-shCon groups, as characterized by EdU assays. Representative images are shown. Scale bars, 100 μm. C-D LV-shANGPTL4 cell-derived CM decreased the migration of HUVECs, as shown using the Transwell assay. Representative images are shown Scale bars, 400 μm. E Compared to LV-shANGPTL4 CM, CM collected from cells in the LV-shCon group increased HUVEC adhesion. Representative images of attached cells are shown. Scale bars, 100 μm