Fig. 6

ANGPTL4 promotes OC progression via CDH5. A Top network identified by IPA. Gene signatures of metastatic sites and primary sites of OC. B CDH5 protein levels in HO8910 and SKOV3 cells with stable ANGPTL4 KD were analyzed by Western blotting. C CDH5 mRNA levels in HO8910 and SKOV3 cells with stable ANGPTL4 KD were analyzed by RT–qPCR. D Representative immunohistochemical staining of ANGPTL4 and CDH5 in 97 OC tissues. Correlation of the expression levels of ANGPTL4 and CDH5 in 97 OC tissues. (r = 0.1643, p < 0.0001), Scale bars, 200 μm. E The effect of gene vector delivery on CDH5 mRNA levels was assessed by RT-PCR; the bar graph shows the mean fold change over data from the control (n = 3). F Cell migration and invasion assays were performed using 24-well Transwell plates at 24 h after plating. These data are from at least three independent experiments and are shown as the means ± SD. One-way ANOVA. Scale bars, 400 μm. G The indicated cells stably transfected with control or ANGPTL4 shRNA were used to analyze protein levels by Western blotting. H-I. The indicated cells stably transfected with control or ANGPTL4 shRNA were used to analyze the mRNA levels of the indicated molecules by qRT-PCR