Fig. 7

ANGPTL4 upregulation of CDH5 expression is modulated by ETV5. A Results of Co-IP analysis of ANGPTL4 and CDH5 levels detected by Western blotting. B A heatmap showing differentially regulated genes in SKOV3 cells and HO8910 cells transfected with control or ANGPTL4 shRNA. The color scheme is shown beside the data. C ETV5 protein levels in HO8910 and SKOV3 cells with stable ANGPTL4 KD were analyzed by Western blotting. D ChIP-qPCR analysis of ETV5 binding at loci 1, 2, 3, 4, and 5. The means ± SDs of triplicate experiments are shown. *P < 0.05, **P < 0.01, ***P < 0.001. E CDH5 protein levels were determined by Western blotting after ETV5 expression was downregulated. F The protein levels of ANGPTL4, ETV5, CDH5, p-AKT and MMP9 in LV-shANGPTL4 SKOV3 cells were analyzed by Western blotting