Fig. 1

Identification of human theca cells. A Representative images of IHC staining for CYP17A1 in ovarian tissues collected from follicle fluid during oocyte retrieval. In the control group, samples were treated in the same manner, but normal rabbit IgG was used instead of the primary antibodies. Scale bar: 100 μm. B Representative images of IF staining for CYP17A1 (green) and FSHR (green) in the isolated cells. In the control group, samples were treated in the same manner, but PBS was used instead of the primary antibodies. Nuclei were stained with DAPI (blue). Scale bar: 250 μm