Fig. 4

Expression of AREG in PCOS granulosa cells and theca cells. A The concentration of AREG in follicle fluid of PCOS patients and normal participants were measured by ELISA. B The mRNA levels of AREG in granulosa cells of PCOS patients and normal participants were measured by quantitative real-time PCR. C The mRNA levels of AREG, EGFR, and LHCGR in theca cells of PCOS patients and normal participants cultured for 6 h with or without the presence of LH (400 mIU/mL) were measured by quantitative real-time PCR. D The mRNA levels of AREG in both granulosa cells and theca cells were measured by quantitative real-time PCR. In A and B, data are displayed as mean ± SEM (n = 6 per group); in C and D, each experiment was repeated three times. *P < 0.05, ** P < 0.01, ***P < 0.001