Fig. 1

Reduced fertility in HMGB2-KO mice Body weight (A) and ovary weight (B) of 2- and 6-month-old WT and HMGB2-KO mice. Macrophotography of WT and HMGB2-KO mouse ovaries at 2 and 6 months of age (C). Arrows indicate ovaries of HMGB2-KO mouse. Western blotting of HMGB2 in mouse ovaries at 2- and 6-months-old WT and KO (D). Number of pups from WT and HMGB2-KO mice (E). The average number of fertilized oocytes in WT and HMGB2-KO mice (F). IVF using oocytes obtained from WT and HMGB2-KO mice at 24 and 48 h (G). Successfully fertilized oocytes at 24 and 48 h obtained from HMGB2-KO mice are indicated by an arrow and arrowhead, respectively. *P < 0.05, **P < 0.01 Student’s t-test (two tailed). Scale bar 100 µm.