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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Integrated analysis of single-cell RNA-seq and bulk RNA-seq unveils heterogeneity and establishes a novel signature for prognosis and tumor immune microenvironment in ovarian cancer

Fig. 1

Analysis of single-cell RNA sequencing. A Post quality control filtering of each sequenced cell, which was plotted in violin plots to display their number of RNA features (nFeature_RNA) and absolute UMI counts (nCount_RNA). B Correlation analysis between sequencing depth and mitochondrial gene sequences and total intracellular sequences. C 10239 non-variable genes and 1500 variable genes were analyzed. D PCA based on scRNA-seq data. E 15 PCs were identified as the criteria of P < 0.5. F Heatmap illustrated the expression patterns of the top ten markers in individual cells of each cluster by Seurat analysis. G Cells were clustered into 12 types via tSNE analysis. H Pseudotime and trajectory analysis

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Journal of Ovarian Research

ISSN: 1757-2215

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