Fig. 1
FOXD3 attenuates the chemoresistance of OC cells. A mRNA expression of FOXD3 determined by RT-qPCR in A2780S, SKOV3, IOSE80, A2780CP, and SKOV3/CDDP cell lines. B Western blot analysis of FOXD3 protein in A2780S, SKOV3, IOSE80, A2780CP, and SKOV3/CDDP cell lines. C IC50 values of A2780S and A2780CP cells treated with CP at different concentration measured by CCK-8 assay. A2780CP and A2780S cells were treated with CP and then transfected with oe-FOXD3 and si-FOXD3, respectively. D, mRNA expression of FOXD3 determined by RT-qPCR in A2780S and A2780CP cells. E Viability of CP-treated A2780S and A2780CP cells measured by CCK-8 assay. F Colony formation of CP-treated A2780S and A2780CP cells measured by colony formation assay. G Quantitative analysis of panel (F). H Flow cytometric analysis of the apoptosis of CP-treated A2780S and A2780CP cells. * p < 0.05, compared with IOSE80 cells or oe-NC + CP-treated A2780CP cells; # p < 0.05, compared with A2780S cells or si-NC + CP-treated A2780S cells; & p < 0.05, compared with SKOV3 cells. The experiment was conducted three times independently