Fig. 3

miR-335 targets DAAM1 and inhibits its expression in OC cells. A Venn diagram analysis of downstream target genes of miR-355 predicted by the miRDB, mirDIP, and TargetScan databases. The three circles in the figure represent the prediction results of three databases, and the central represents the intersection of three groups of data. B Interaction analysis of the candidate miRNA target genes; each circle in the figure represents a gene, and the line between circles indicates interaction between two genes; the more interacted genes exist in a gene, the higher the degree value, and the higher the core degree in the interaction network. C Degree values of core genes. The abscissa represents the degree value, and the ordinate represents the gene name. D KM survival curve indicating the correlation between the expression of DAAM1 and the survival rate of patients with OC. Red indicates patients with high expression of DAAM1 and blue indicates those with low expression of DAAM1. E KEGG enrichment analysis of candidate genes. The abscissa represents GeneRatio, the ordinate represents the entry identifier, the color represents enrichment p value, and the size represents the number of enriched genes in the identifier. F miR-335 targeted binding sites in the 3’UTR of DAAM1 predicted by the TargetScan database. G DAAM1 mRNA expression determined by RT-qPCR in A2780S, IOSE80 and A2780CP cell lines. H Western blot analysis of DAAM1 protein in A2780S, IOSE80 and A2780CP cell lines. I Binding of miR-335 to DAAM1 confirmed by dual-luciferase reporter assay in HEK-293 T cells. J miR-335 expression determined by RT-qPCR in miR-335 mimic-transfected A2780CP cells. K DAAM1 expression determined by RT-qPCR in miR-335 mimic-transfected A2780CP cells. L Western blot analysis of DAAM1 protein in miR-335 mimic-transfected A2780CP cells. * p < 0.05, compared with IOSE80 cells, or mimic NC-transfected A2780CP cells; # p < 0.05, compared with A2780S cells. The experiment was conducted three times independently