Fig. 4

FOXD3 decelerates the growth and chemoresistance of OC cells via facilitation of the miR-335-mediated DAAM1 inhibition. A FOXD3, miR-335 and DAAM1 expression determined by RT-qPCR in A2780CP cells transfected with oe-FOXD3 or combined with miR-335 inhibitor. B Western blot analysis of DAAM1 protein in A2780CP cells transfected with oe-FOXD3 or combined with miR-335 inhibitor. CP-treated A2780CP cells were transfected with si-DAAM1 or combined with si-FOXD3, while CP-treated A2780S cells were transfected with oe-DAAM1 or combined with oe-FOXD3. C Viability of A2780S and A2780CP cells measured by CCK-8 assay. D Colony formation of A2780S and A2780CP cells measured by colony formation assay. E Flow cytometric analysis of the apoptosis of A2780S and A2780CP cells. * p < 0.05, compared with A2780CP cells transfected with oe-NC + inhibitor NC or treated with si-NC + CP or A2780S cells treated with oe-NC + CP; # p < 0.05, compared with A2780CP cells transfected with oe-FOXD3 + inhibitor NC or treated with si-DAAM1 + CP, or A2780S cells treated with oe-DAAM1 + CP. The experiment was conducted three times independently