Fig. 5

DAAM1 knockdown blunts myosin II activation in CP-resistant OC cells. A MLC2 expression and the extent of MLC2 phosphorylation determined by Western blot analysis in A2780S, A2780CP and IOSE80 cells. B MLC2 expression and the extent of MLC2 phosphorylation determined by Western blot analysis in blebbistatin-treated A2780CP cells. C Viability of blebbistatin-treated A2780CP cells measured by CCK-8 assay. D Colony formation of blebbistatin-treated A2780CP cells measured by colony formation assay. E Flow cytometric analysis of the apoptosis of blebbistatin-treated A2780CP cells. F DAAM1 mRNA expression determined by RT-qPCR in A2780CP cells treated with si-DAAM1. G DAAM1 and MLC2 protein expression as well as the extent of MLC2 phosphorylation determined by Western blot analysis in A2780CP cells treated with si-DAAM1. * p < 0.05, compared with IOSE80 cells, or A2780CP cells treated with DMSO or si-NC; # p < 0.05, compared with A2780CP cells. The experiment was conducted three times independently