Fig. 6

FOXD3/miR-335/DAAM1 affects chemoresistance of OC cells by regulating myosin II. CP-treated A2780CP cells were treated with blebbistatin or combined with si-FOXD3. A mRNA expression of FOXD3 and DAAM1 as well as miR-335 expression determined by RT-qPCR in A2780CP cells. B DAAM1 and MLC2 protein expression as well as the extent of MLC2 phosphorylation determined by Western blot analysis in A2780CP cells. C A2780CP cell viability measured by CCK-8 assay. D Colony formation of A2780CP cells measured by colony formation assay. E Flow cytometric analysis of A2780CP cell apoptosis. * p < 0.05, compared with A2780CP cells treated with DMSO + si-NC + CP; # p < 0.05, compared with A2780CP treated with blebbistatin + si-NC + CP. The experiment was conducted three times independently