Fig. 7

FOXD3 increases the sensitivity of OC cells to CP in vivo. Mice were treated with CP or combined with oe-FOXD3. A Expression of FOXD3, miR-335 and DAAM1 determined by RT-qPCR in mouse tumor tissues. B DAAM1 and MLC2 protein expression as well as the extent of MLC2 phosphorylation determined by Western blot analysis in mouse tumor tissues. C Positive expression of pMLC2 in mouse tumor tissues determined by Immunohistochemical staining (scale bar = 50 μm). D, Representative images showing xenografts in nude mice. E Tumor volume of mice measured every five days. F Tumor weight of mice. G HE staining of mouse tumor tissues (scale bar = 25 μm). # p < 0.05, compared with mice treated with oe-NC + CP. N = 10 for mice following each treatment