Fig. 5

Morphological appearance in ovaries of rats from three groups (n = 10 rats/group). A-C) Survey view showing ovaries, A-C represents the CON group, LE group and EA group respectively (magnification40×; distance bars, 50 μm); A1-C1) Higher magnification image of A-C. (magnification 100×; distance bars, 100 μm); A2-C2), Magnification 400×; distance bars, 20 μm. A2) Higher-power view of rectangular area in A1 shows a secondary follicle with corona radiata and zona pellucida, the granulosa cell layer(GL) and theca cell layer (TL) appear normal; B2) Higher-power view of rectangular area in B1 shows a cystic dilated follicle. The cyst wall has a thick, vascularized layer of luteinized cells and thin granulosa cell layer; C2) Higher-power view of rectangular area in C1 shows a cystic follicle and a secondary follicle. PF, primary follicles. SF, secondary follicle. CF, cystic follicle. CL, corpus luteum. GL, granulosa cell layer. TL, theca cell layer. ZP, zona pellucida. CR, corona radiata. PO, primary oocyte