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Fig. 8 | Journal of Ovarian Research

Fig. 8

From: Effects of electroacupuncture on the kisspeptin-gonadotropin-releasing hormone (GnRH) /luteinizing hormone (LH) neural circuit abnormalities and androgen receptor expression of kisspeptin/neurokinin B/dynorphin neurons in PCOS rats

Fig. 8

Coexpression of AR, ERα, and Kiss1 in the hypothalamus arcuate nucleus (ARC) of rats from the CON, LE, and EA groups (n = 5 rats/group). A The coexpression of AR, ERα, and Kiss1 in the hypothalamic ARC of rats was assessed via triple-label immunofluorescence (400×). Scale bars: 50 μm. B a: A lower magnification image showing the entire ARC and median eminence (150×). Rectangular frame represents ARC analyzed in A.3v:Scale bar: 100 μm. b: A higher magnification image showing the rectangle area (400×), Scale bars: 20 μm. C The number of AR-, ERα-, and Kiss1-positive cells in the hypothalamic ARC of rats in each group. D The number of AR- and ERα-positive cells coexpressed with Kiss1 cells in the hypothalamic ARC of rats in each group. Values shown are mean ± standard deviation. Error bars represent standard deviation. # P < 0.05 vs. CON group; * P < 0.05 vs. LE group

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