Fig. 9

Protein expression levels of Kiss1, Kiss1r, AR, and ERα in the hypothalamic arcuate nucleus (ARC) of rats from the CON, LE, and EA groups (n = 5 rats/group). A–D The bar graph showing the ratio of Kiss1, Kiss1r, AR, and ERα band intensity to GAPDH band intensity in the ARC of rats from three groups. E Representative western blot of Kiss1, Kiss1r, AR, ERα, and GAPDH; Error bars represent standard deviation. # P < 0.05 vs. CON group; * P < 0.05 vs. LE group