Table 1 Primer sequences of the targeted genes in rats
Targeted genes | Forward and reverse primers (5′ → 3′) | Amplicon size (bp) | Accession number |
|---|---|---|---|
Kiss1 | TGTCAGCCTACAACTGGAACTCC | 147 | NM_181692.1 |
TTGCACAAGTCTAGAAGCTCCCT | |||
Kiss1r | TAGTCGGGAACTCACTGGTCATC | 241 | NM_001301151.1 |
CTCATGGCTGTCAAAGTGGCA | |||
Ar | CTGATTCCTTTGCTGCCTTGT | 188 | NM_012502.2 |
ATTAGTGAAGGACCGCCAACC | |||
Esr1 | TAGTCGGGAACTCACTGGTCATC | 178 | NM_012754.3 |
CTCATGGCTGTCAAAGTGGCA | |||
Gapdh | CTGGAGAAACCTGCCAAGTATG | 138 | NM_017008.4 |
GGTGGAAGAATGGGAGTTGCT |