Fig. 1

Experimental process and the endocrine function of the OTT. a Schematic illustration of the animal experiment. (a₁) Transplantation of ovarian tissue into the peritoneum of ovariectomised mice. (a₂) Ovarian tissue in the peritoneal pouch after transplantation, implanted ovarian tissue is shown in the red box. (a₃) Images of ovarian tissue haematoxylin and eosin staining. b Changes in the levels of serum E₂, FSH, LH and inhibin in the four groups. c Representative images of the four groups of vaginal cytology are as follows: SHAM, OVX, E₂ and OTT. * indicates significance P < 0.05 compared to normal mice; # indicates significance P < 0.05 compared to OVX mice; & indicates significance P < 0.05 compared to E₂ mice as determined by a two-way ANOVA followed by Tukey’s post hoc analysis