Fig. 4

TP induces cellular apoptosis of granulosa cell. After intervening AR expression, CCK-8 (A, * P < 0.05 vs. Control), Hoechst (B, apoptotic bodies are indicated by yellow arrows, 20 ×) and Flow cytometry (C, ** P < 0.01 vs. Control, * P < 0.05 vs TP) were detected the viability and apoptosis respectively. To further verify the effect of klotho in TP-induced GCs, after silence klotho, also used Hoechst (D, apoptotic bodies are indicated by yellow arrows, 20 ×) and Flow cytometry (E, *** P < 0.001 vs. Control, ns P > 0.05 vs. TP, ** P < 0.01 vs. TP) to detected the GCs apoptosis