Fig. 5

TP increases AR and klotho contents in vitro. GCs were cultured with TP (0—100 nM, 24 h) changes in AR and klotho expression were assessed by Immunocytochemistry (A and B, respectively). Western blot (C and D, respectively) was used to detect the expression of AR and klotho in TP (100 nM, 24 h)-induced GCs. The expression of AR and klotho were high-express in TP, this response was attenuated by flutamide