Fig. 3

GIMAP7 shRNA relieves ovarian apoptosis and oxidative stress in PCOS rats. A-F Sprague–Dawley rats (n = 6 per group) were injected with DHEA for constructing PCOS animal models, and then injected with lentivirus carrying shNC or shGIMAP7. A The apoptosis of ovarian tissues was detected using TUNEL staining. Representative images of TUNEL are shown. B The protein expression of c-caspase-3 was detected using western blot. The GSH levels in ovaries (C). The MDA levels in serum (D) and ovaries (E). The SOD levels in serum (F) and ovaries (G). Differences among multiple groups were analysed using a one-way analysis of variance.*P < 0.05, **P < 0.01, ***P < 0.001