Fig. 4

GIMAP7 siRNA promotes the proliferation of ovarian granulosa cells. A-E Ovarian granulosa cell line KGN was transfected with siNC or GIMAP7 siRNAs. The GIMAP7 mRNA (A) and protein (B) expression in KGN cells was detected using qRT-PCR and western blotting. The cell viability (C), proliferation (D), and cell cycle (E) were detected using CCK-8 assay, EdU staining, and flow cytometry. All experiments repeated three times. Images show only one representative result. Differences among multiple groups were analysed using a one-way or two-way analysis of variance. *P < 0.05, **P < 0.01, ***P < 0.001