Fig. 5

GIMAP7 siRNA inhibits the apoptosis and oxidative stress of KGN cells. A-G Ovarian granulosa cell line KGN was transfected with siNC or GIMAP7 siRNAs. A The KGN cell apoptosis was detected using flow cytometry. B The c-caspase-3 protein of KGN cells were measured using western blotting. The oxidative stress biomarkers ROS (C, D), MDA (E), GSH (F), and SOD (G) in KGN cells. All experiments repeated three times. Images show only one representative result. Differences among multiple groups were analysed using a one-way analysis of variance. *P < 0.05, **P < 0.01, ***P < 0.001