Fig. 6

GIMAP7 inhibits the sonic hedgehog signalling pathway in KGN cells. A KEGG analysis showed that GIMAP7 inhibited the sonic hedgehog signalling pathway. B The genes enriched in the sonic hedgehog signalling pathway. C-F Ovarian granulosa cell line KGN was transfected with siNC or GIMAP7 siRNAs. The mRNA expression of SHH (C), SMO (D), and Gli1 (E) in KGN cells was detected using qRT-PCR. F The protein expression of SHH, SMO, and Gli1 in KGN cells was detected using western blotting. G Sprague–Dawley rats were injected with DHEA for constructing PCOS animal models, and then injected with lentivirus carrying shNC or shGIMAP7. The protein expression of SHH, SMO, and Gli1 was detected using western blotting. H Diagram of GIMAP7 inhibiting the sonic hedgehog signalling pathway to promote PCOS progress. All experiments repeated three times. Images show only one representative result. Differences among multiple groups were analysed using a one-way analysis of variance. **P < 0.01, ***P < 0.001