Fig. 7

Inhibition of the SHH signalling pathway reduced the effects of GIMAP7 silencing on KGN cells. A-C Ovarian granulosa cell line KGN was transfected with siNC or GIMAP7 siRNAs, and were treated with 5 μM cyclopamine for 24 h. The proliferation (A), apoptosis (B), and ROS level (C) of KGN cells were detected using EdU staining, flow cytometry, and DCFDA staining. All experiments repeated three times. Images show only one representative result. Differences among multiple groups were analysed using a one-way analysis of variance.*P < 0.05, **P < 0.01, ***P < 0.001