Table 1 List of scRNAseq studies on adult mice and human ovaries
Authors and study details | Salient findings |
|---|---|
Fan et al. 2019 Nature Communications [4] Human ovaries | scRNAseq studies on human adult ovaries to develop molecular signature of growing and regressing follicular populations. Study identified different types of granulosa and theca cells, mixture of adaptive and innate immune cells, endothelial and smooth muscle cells. All cell processing was done on cells collected by centrifuging at 160 g |
Wagner et al. 2020 Nature Communications [1] Human ovaries | Single-cell transcriptomes and cell surface antigen profiles of over 24,000 cells from ovarian cortex samples from 21 patients. Identified transcriptional profiles of six main cell types; oocytes, granulosa cells, immune cells, endothelial cells, perivascular cells, and stromal cells. Adult human ovarian cortex consists of six main cell types and do not harbor germline stem cells Cells collected by centrifuging at 300 g |
Wang et al. 2020 Cell [5] Monkey ovaries | scRNAseq studies on young and aged ovaries of monkeys identified stromal cells, smooth muscle cells, granulosa cells, endothelial cells, NK cells, macrophages and oocytes with distinct gene-expression signatures Aging was associated with the disturbance of antioxidant signaling specific to early-stage oocytes and granulosa cells. Oxidative damage emerged as a crucial factor in ovarian functional decline with age. Also, inactivated antioxidative pathways, increased reactive oxygen species, and apoptosis were observed in granulosa cells from aged women Cells collected by centrifuging at 1000 rpm |
Morris et al. 2022 eLife [6] https://doi.org/10.1101/2022.02.08.479522 Mouse ovaries | scRNAseq was used to evaluate the transcriptome of > 34,000 cells of the adult mouse ovary and describe transcriptional changes across estrus cycle and other reproductive states. Major clusters included granulosa cells, stromal cells, endothelial cells, ovary surface epithelial cells, immune cells and oocytes Dynamic mRNA expression in murine ovaries is reported across the estrus cycle and other reproductive states. Extends our understanding of the diversity of cell types in the adult ovary Speed used for centrifuging cells not mentioned |
Wang et al. 2022 Research Square [7] https://doi.org/10.21203/rs.3.rs-1624864/v1 Human ovaries | Eight different cell types were detected by scRNAseq studies on human ovaries of different ages including granulosa cells, theca and stromal cells, smooth muscle cells, endothelial cells, monocytes, Natural Killer cells, T lymphocytes scRNAseq results picked up cellular senescence and inflammatory changes in aged ovaries. DNA damage genes were upregulated while DNA repair genes were downregulated in the oocytes Cells collected by centrifuging at 1200 rpm |
Shikanov lab https://event.on24.com/wcc/r/3857131/C1C9EE36B2D21B71D4E49A951201C341?partnerref=WEB Human ovaries | Single cell sequencing and spatial transcriptomics to create a tissue and cell atlas of the human ovary. Analysis of 21,198 cells uncovered four primary cell types found in the human ovary: immune cells, endothelial cells, pericytes, and stromal cells Speed used for centrifuging cells not mentioned |