Fig. 1

Up-regulation of NUF2 was associated with poor survival in OC. A High NUF2 expression was found in OC patients based on oncomine datasets. B Immunohistochemical evaluation of NUF2 expression in HGSOC tissues and adjacent normal tissues. Morderate or strong NUF2 expression was confirmed in HGSOC tissues, and negative or weak expression in adjacent normal tissues. Scale bars, 500 μm. C The expression levels of NUF2 in OC cell lines were analyzed by western blot. Quantification of NUF2 expression relative to GAPDH were performed. The experiment was repeated 3 times. In each time, the levels of NUF2 relative to GAPDH were normalized to the Human Ovarian Surface Epithelial Cells (HOSEpiC) group that was arbitrarily set as 1. Values are expressed as the mean ± SE. 2-tailed Student t test was used for C. NUF2 protein was overexpressed in HEY and SKOV3 cells compared with Human Ovarian Surface Epithelial Cells (HOSEpiC). *P < 0.05. D ScRNA-seq profiling of four primary carcinomas, two relapsed, and two metastatic HGSOCs indicating higher NUF2 expression in malignant epithelial cells (C0 and C2) than other cell types. E, F Kaplan–Meier (KM) plots indicated the correlation of NUF2 expression with overall survival (OS) and progression-free survival (PFS) of OC patients. Patients with higher NUF2 had poorer OS and PFS. G, H Subgroup analyses of survival associated with NUF2 in OC patients