Fig. 3

Effect of NUF2 silencing on OC cell proliferation and apoptosis. A Western blot was used to validate the efficiency of NUF2 knockdown by small interfering RNA (siRNA) treatment. Quantification of NUF2 expression relative to GAPDH were performed. The result was obtained with 3 independent experiments. In each experiment, the protein levels of NUF2 related to GAPDH were normalized to control group that was arbitrarily set as 1. *P < 0.05. B The CCK8 assay was conducted to detect the proliferation of the HEY and SKOV3 cells treated with si-control or si-NUF2 respectively, and knockdown of NUF2 significantly impaired the proliferation of cells. *P < 0.05. C Colony formation was conducted to detect the viability of the HEY and SKOV3 cells treated with si-control or si-NUF2 respectively, and knockdown of NUF2 significantly impaired the proliferation of cells. The no. of colonies or no. of cells per colony measured in three independent experiments were statistically analyzed. In each experiment, the no. of colonies or no. of cells per colony were normalized to control group that was arbitrarily set as 1. *P < 0.05. D Flow cytometry assay was used to detect the apoptosis of SKOV3 cells treated with si-control or si-NUF2. The result was obtained with 3 independent experiments. Values are expressed as the mean ± SE. 2-tailed Student t test was used for D. E Western blot analysis for proteins involved in apoptosis revealed that cleaved-caspase3 and cleaved-PARP were considerably increased after siRNA treatment. The total protein expressions of caspase3 and PARP remained unchanged. Experiments were repeated three times. In each experiment, the protein levels of cleaved-caspase3 or cleaved-PARP related to the total caspase3 or PARP were normalized to the control group that was arbitrarily set as 1