Fig. 5

NUF2 physically associates with HNRNPA2B1. A IP assays were conducted in SKOV3 cells transfected with si-control; IgG was used as control. B Immunofluorescence staining showed that HNRNPA2B1 (green) co-localized with NUF2 (pink) in the SKOV3 cells. DAPI was used to stain the nuclear. Scale bars = 10 μm. C Representative IHC staining of HGSOC tumors for HNRNPA2B1 and NUF2 expression. Scale bars = 500 μm. IHC results revealed that expression of HNRNPA2B1 and NUF2 was positively interrelated in OC tissues as shown in the histogram (right). D GEPIA was applied to investigate the correlated expression between HNRNPA2B1 and NUF2. E Silencing HNRNPA2B1 decreased the mRNA expression of NUF2 by RT-qPCR assay. The experiment was repeated 3 times. In each experiment, HNRNPA2B1 or NUF2 mRNA levels were assessed by RT-qPCR and normalized to GAPDH. The HNRNPA2B1 or NUF2 mRNA levels were normalized to the control group that was arbitrarily set as 1.*P < 0.05. F Silencing HNRNPA2B1 decreased the protein expression of NUF2 by western blot. Quantification of HNRNPA2B1 and NUF2 expressions relative to GAPDH were shown. The experiment was repeated 3 times. In each time, the other groups were normalized to the control group that was arbitrarily set as 1. *P < 0.05