Fig. 6

HNRNPA2B1-NUF2 active the PI3K/AKT/mTOR signaling pathway. A Scatter plots of HNRNPA2B1 and genes involved in PI3K/AKT/mTOR signaling pathways. B GSEA of enriched pathways in 180 samples exhibited high HNRNPA2B1 expression and in the remaining 180 samples exhibited low HNRNPA2B1 expression, which indicated that the high HNRNPA2B1 expression was related to PI3K/AKT/mTOR signaling pathway. P < 0.05. C Scatter plots of NUF2 and genes involved in PI3K/AKT/mTOR signaling pathways. D Enriched pathway found by the GSEA using MsigDB. PI3K/AKT/mTOR signaling pathway is enriched in OC samples with highly expressed NUF2. P < 0.05. E, F Western blot was conducted to analyze the expressions of PI3K/AKT/mTOR signaling pathway-related genes. The result was obtained by 3 independent experiments. In each experiment, the protein levels of PIK3CA or PIK3CB relative to GAPDH were normalized to the control group that was arbitrarily set as 1. The protein levels of p-AKT or p-mTOR related to the total AKT or mTOR were normalized to the control group that was arbitrarily set as 1. HNRNPA2B1 or NUF2 knockdown reduced the expression levels of PIK3CA, PIK3CB, p-AKT and p-mTOR. *P < 0.05