Fig. 1

The effects of the GPER-specific agonist (G-1) or antagonist (G-15) and/or bisphenol A (BPA) and bisphenol (BPS) on ovine granulosa cells (GC) steroidogenesis. The progesterone (A) and oestradiol (B) concentrations were determined in culture medium after 48 h of culture in complemented serum-free McCoy’s 5A media in the presence or absence (control) of BPA or BPS at 10 or 50 μM, and/or G-15 at 10 µM or G-1 at 1 μM. The results are expressed as the mean ± standard error of the mean of 10 independent cultures. Each condition was performed in duplicate and normalised to the control condition of each culture experiment. Bars with different superscripts indicate a significant difference (p ≤ 0.05). The actual control values were 37.07 ± 4.68 ng progesterone/mg protein (A) and 52.04 ± 2.21 pg oestradiol/mg protein B