Fig. 2

Differentially expressed genes (DEG) in ovine granulosa cells (GC) treated with bisphenol A (BPA), bisphenol S (BPS) or the GPER-specific agonist (G-1). After 1 h culture in complemented serum-free McCoy’s 5A media in the presence or absence (control) of BPA or BPS at 50 μM, or G-1 at 10 μM, six biological replicates were analysed with RNA-sequencing, and DEG were identified (padj ≤ 0.05). The Venn diagram (A) shows common and specific DEG from four comparisons: control versus BPA, control versus BPS, BPA versus BPS, and Control versus G-1. The heatmap (B) shows hierarchical clustering of DEG. The ordered list of heatmap genes have been annotated in Supplementary Table S3. Principle component analysis of gene expression in GC treated or not with BPA, BPS or G1 performed on expression values of 24 differential genes C