Fig. 3

Functional analysis of differentially expressed genes (DEG) from RNA-sequencing of ovine granulosa cells (GC). After 1 h of treatment in the presence or absence (control) of bisphenol A (BPA) or bisphenol S (BPS) at 50 μM, GC (six replicates per condition) were analysed with RNA-sequencing to obtain a list of DEG (p ≤ 0.05). The global clustering heatmap plot of functional sets of gene ontology (GO; p ≤ 0.01) terms was obtained by using ViSEAGO. From left to right are: the major processes, the cluster name, a heatmap of GO term counts from functional enrichment tests and a dendrogram based on Wang’s semantic similarity distance and Ward’s clustering criterion