Table 1 Ovine oligonucleotide primer sequences used in this study
Abbrev | Name | Gene ID | Forward (5ʹ → 3ʹ) | Reverse (5ʹ → 3ʹ) | Size (bp) | E (%) |
|---|---|---|---|---|---|---|
ACTB | β-actine | NM.001009784.3 | CCAGCACGATGAAGATCAAG | ACATCTGCTGGAAGGTGGAC | 102 | 109.6 |
ARRDC4 | Arrestin domain containing 4 | XM_015101774.3 | GTTTTGGCAGCAGAAACTCC | GCAAACATAGGACAGCACGA | 153 | 107.7 |
CHAC1 | ChaC glutathione specific gamma-glutamylcyclotransferase 1 | XM_012181220.4 | GTGGTGACCCTCCTTGAAGA | AATGCCTTGAGTGGTTGGTC | 182 | 106.4 |
EGR3 | Early growth response 3 | XM_027964329.2 | ACCTAGCAAGACCCCTCTCC | GCGGATGTGAGTGGTAAGGT | 187 | 102.4 |
JUN | Jun proto-oncogene, AP-1 transcription factor subunit | XM_004002020.5 | ACGACCTTCTACGACGATGC | TGAGGAGGTCGGAGTTCTTG | 166 | 106.8 |
KLF9 | Kruppel like factor 9 | XM_042243358.1 | CCCAGTGTCTGGTTTCCATT | CTGGATGGGTCGGTACTTGT | 197 | 106.2 |
KLF10 | Kruppel like factor 10 | XM_027973155.2 | CCTTCCTCAGCGAAAGTCAC | CAGGCGAACTTCTTCTCACC | 238 | 98.9 |
KLF11 | Kruppel like factor 11 | XM_004005679.5 | TCATTGCATCCAGTCAGAGC | TGCAGCTGAAAGGCTTCTCT | 156 | 105.8 |
LIF | LIF interleukin 6 family cytokine | XM_042234817.1 | TGGAGCTGTATCGCATCATC | TGACAGCCCAGCTTCTTCTT | 250 | 100.7 |
LSMEM1 | Leucine Rich Single-Pass Membrane Protein 1 | XM_042249200.1 | GAAACGTTCTTCCCAGGACA | ATCACGAAGGAAACCAGTGC | 243 | 91.2 |
NOTCH1 | Notch Receptor 1 | XM_027966164.2 | CGATGAGTGTGAGTCGAACC | AGGCAGTTGCATTTGTACCC | 192 | 102.4 |
NR4A1 | Nuclear receptor subfamily 4 group A member 1 | XM_042246696.1 | GGGGGAGGGAGAGAGCTATT | ACCATAATGCTGGCAGGAAG | 187 | 103.1 |
RPL19 | Ribosomal protein L19 | AY158223.1 | CACAAGCTGAAGGCAGACAA | TGATGATTTCCTCCTTCTTGG | 130 | 103.9 |
TXNIP | Thioredoxin interacting protein | GAAI01004920.1 | GCCTCTGGGAACATCTTTCA | TTCGTGCAGAGACAGACACC | 248 | 99.4 |