Fig. 2

Expression of genes and proteins involved in insulin signaling cascade in human luteinized granulosa cells isolated from control, PCOS-IR, PCOS-NIR and treated with swertiamarin and metformin. A Western blot image for INSR-β, pIRS(ser307), PI(3)K, pAkt, PKC-ζ, ERK1/2, pP38MAPK and PPAR γ from PCOS-IR. B Densitometric analysis for INSR-β, pIRS (ser307), PI(3)K, pAkt, PKC-ζ, ERK1/2, pP38MAPK and PPAR γ from PCOS-IR. C Western blot image for INSR-β, pIRS(ser307), PI(3)K, pAkt, PKC-ζ, ERK1/2, pP38MAPK and PPAR γ from PCOS-NIR. D Densitometric analysis for INSR-β, pIRS (ser307), PI(3)K, pAkt, PKC-ζ, ERK1/2, pP38MAPK and PPAR γ from PCOS-NIR. E mRNA expression of SREBP1c, FAS, ACC-1, CPT-1 genes involved in fatty acid metabolism by qRT-PCR PCOS-IR. F. mRNA expression of SREBP1c, FAS, ACC-1, CPT-1 genes involved in fatty acid metabolism by qRT-PCR PCOS-NIR. * P < 0.05, ** P < 0.01 vs. C, ^# P < 0.05, ^## P < 0.01, ^### P < 0.001 vs. PCOS-IR or PCOS-NIR, ^@ P < 0.05 vs. PCOS-IR + S, n = 8 control, n = 8 PCOS-IR and n = 8 PCOS-NIR