Fig. 4

Steroidogenic profile of human luteinized granulosa cells isolated from control, PCOS-IR and PCOS-NIR and treated with swertiamarin and metformin. A mRNA expression of StAR, CYP11A1, 3β-HSD, CYP19A1, 17β-HSD genes in PCOS-IR by qRT-FAST PCR B mRNA expression of StAR, CYP11A1, 3β-HSD, CYP19A1, 17β-HSD genes in PCOS-NIR by qRT-FAST PCR. C Protein expression of StAR, CYP11A1, 3β-HSD, CYP19A1, 17β-HSD by Western blot method from PCOS-IR. D Densitometry analysis using β-actin as endogenous control from PCOS = IR. E Protein expression of StAR, CYP11A1, 3β-HSD, CYP19A1, 17β-HSD by Western blot method from PCOS-NIR. F Densitometry analysis using β-actin as endogenous control from PCOS-NIR. * P < 0.05, ** P < 0.01, *** P < 0.001 vs. C, ^# P < 0.05, ^## P < 0.01, ^### P < 0.001 vs. PCOS-IR or PCOS-NIR, ^@ P < 0.05, ^@@ P < 0.01, ^@@@ P < 0.001 vs. PCOS-IR + S. n = 8 control and n = 8 PCOS-IR