Fig. 1

Ovarian engineered constructs transplantation. Ovarian engineered constructs were transplanted into adult female mice (NMRI). A Mice were first anesthetized via intra-peritoneal injection of mixture of a 100 mg/kg of ketamine and 15 mg/kg of xylazine. B The ovaries (dashed circle) were excited, and (C-F) the constructs placed within a small pocket created within anterior abdominal wall peritoneum mesothelium and then sutured (6–0 Nylon) in place. G Gross histology of grafts with cells under anterior abdominal wall peritoneum mesothelium (Arrow) and (H) under the skin (dashed square)