Fig. 6

Stem cells cultured on decellularized ovarian scaffold transplanted to mice and produce estradiol and Amh in vivo. A-D Macroscopic appearance of artificial ovaries with OSCs; SO (A), OSCs + PMSCs; SOP (B), OSCs + BMSCs; SOB (C), OSCs + PMSCs + BMSCs; SOPB (D) 8 weeks after transplantation under sub-peritoneum. (E–H) H&E staining of sections through graft containing follicles-like structures derived from artificial ovaries with OSCs (E), OSCs + PMSCs (F), OSCs + BMSCs (G) and OSCs + PMSCs + BMSCs (H) 8 weeks after transplantation. (I and J) The levels of estradiol and AMH were determined by ELISA in all groups (scale bars: 100 µm). Values are given as mean ± SE. (a-b) Groups followed by the same letter are not significantly different at p < 0.05