Table 1 The Mixed Medium of OGSCs
Name (brand) | Volume | Concentration |
|---|---|---|
β-mercaptoethanol (Sigma) | 0.07 μl | 0.1 mM |
epidermal growth factor (Sigma) | 1 μl | 10 ng/ml |
basic fibroblast growth factor (Sigma) | 1 μl | 1 ng/ml |
mouse glial cell line-derived neurotrophic factor (Sigma) | 1 μl | 40 ng/ml |
mouse leukemia inhibitory factor (Sigma) | 2 μl | 20 ng/m |
non-essential amino acids (Gibco) | 100 μl | 1 mM |
L-glutamine (Sigma) | 100 μl | 2 mM |
sodium pyruvate (Gibco) | 100 μl | 1 mM |
penicillin and streptomycin (Solarbio) | 100 μl | 100 ×  |
FBS (Gibco) | 1 ml | – |
minimum essential medium alpha (MEM-α; Invitrogen) | Up to 10 ml | – |