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Table 2 The following is a summary of information on the primer sequences of related genes:

From: Orexin antagonism and substance-P: Effects and interactions on polycystic ovary syndrome in the wistar rats

Gene

name

Gene

Bank

accession

number

Primer sequence

(5’ -3’)

Amplicon

size (bp)

Star

NM_031558.3

F-TGTACCAAGCGTAGAGGTTC

R-GCATCTCCCCAAAGTGTG

64

Cyp19a1

NM_017085.3

F-GAAAACTTCATTAACGAGAGCC

R-ATGACCAAGTCCACGACAG

52

Gapdh

NM_017008.4

F-AACGACCCCTTCATTGACCT

R-GGTTTCCCGTTGATGACCAG

119

  1. The relative expressions of genes were normalized to the housekeeping gene. F: forward, R: reverse, Star: steroidogenic acute regulatory protein, Cyp19a1: cytochrome P450, family 19, subfamily a, polypeptide 1, Gapdh: glyceraldehydes-3-phosphate dehydrogenase.