Fig. 2

Knockdown of circ-PHC3 inhibits proliferation and growth of OC cells in vivo and in vitro. A qRT-PCR detection of circ-PHC3 expression in OV90 and SKOV3 cells after transfection with negative control (NC) or specific siRNA against circ-PHC3. Data are presented as means ± SD. ***P < 0.001 vs NC. B, C Assessment of cell proliferation via the CCK-8 assay. Data are presented as means ± SD. ***P < 0.001 vs NC. D, E Assessment of OV90 and SKOV3 cell proliferation using the clone formation assay. Data are presented as means ± SD. ***P < 0.001 vs NC. F Representative images of nude mouse xenografts after injection of SKOV3 cells transfected with sh-NC or sh-circ-PHC3. G Data on tumor growth measured every 5 days are presented as means ± SD. ***P < 0.001 vs sh-NC. H Data on tumor weight measured 30 days after grafting are presented as means ± SD. ***P < 0.001 vs sh-NC. I, J Immunohistochemical staining showing the expression of Ki-67 in tumor specimens from the sh-NC and sh-circ-PHC3 groups. Data are expressed as means ± SD. ***P < 0.001 vs sh-NC