Fig. 1

Establishment and characterization of a rat anti-human SPON1 monoclonal antibody (mAb). (A) Topology of SPON1. The squares and circle indicate the thrombospondin domain of SPON1 and the binding region of APP (amyloid-β precursor protein) to SPON1. (B) Amino acid sequences of the N-terminal domains of human and mouse SPON1. The antigen region is highlighted in red. Immunohistochemical (C) and Western blot (D) analyses showing the specificity of the indicated mAbs. 293T cells transfected with SPON1 and empty vectors were subjected to these analyses. Scale bar, 100 μm. (E) ELISA analysis revealing the dose-dependent response of the anti-SPON1 mAb (clone #1) to the SPON1 polypeptide. (F) Immunohistochemical images of SPON1 in the indicated ovarian cancer cell lines. Cell slices were reacted with or without clone #1. Scale bar, 100 μm