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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Extracellular vesicle-microRNAs mediated response of bovine ovaries to seasonal environmental changes

Fig. 1

Experimental Design. Dry beef cows (n = 11) were subjected to synchronization and stimulation for follicular dynamics. Ovum pick-up (OPU) was conducted on all animals in the summer and winter seasons by ultrasound-guided transvaginal follicle aspiration. The follicular fluids (FFs) were collected from each target group (4 biological replicates/group; 8 mL FF/replicate). EVs were then isolated from the FFs using high-speed ultracentrifugation. Isolated EVs were characterized using NanoSight Tracking Analysis, western blot for EV and cellular marker proteins, and transmission electron microscopy. Total RNA was isolated from FF-EVs and small-RNA library preparation and RNAseq (NextSeq500; Illumina) were performed

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Journal of Ovarian Research

ISSN: 1757-2215

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