Fig. 6

Localization and distribution of Cx43 in primary hGL cells. (A and B) Primary hGL cells were pretreated with DMSO, 10 µM AG1478 (A) or 10 µM sotrastaurin (B) for 40 min, and the cells were then treated with 50 ng/ml BTC for an additional 15 min. The localization and distribution of phosphorylated Cx43 protein were examined by immunofluorescence microscopy. Arrow indicates the position of the phosphorylated Cx43 protein. Cell nuclei were stained with DAPI. The scale bar represents 50 μm. Magnification, 400×. The results are expressed as the mean ± SEM of at least three independent experiments. Values marked with different letters were significantly different (P < 0.05)