Fig. 2
From: BCAS2 regulates granulosa cell survival by participating in mRNA alternative splicing
Bcas2 knockout in granulosa cells at 7 dpp ovaries. A Schematic illustration of deletion of Bcas2 exons by injecting tamoxifen. B The WT, Bcas2F/+; Foxl2-CreERT2, and Bcas2F/F; Foxl2-CreERT2 genotypes were detected by common PCR. C, D Real-time PCR and Western blot assays of the knockout efficiency of Bcas2 at 7 dpp. T tests were performed to compare each group, * P < 0.05. Each test had at least three independent replications. E Expression of BCAS2 in the PfGC-Bcas2+/+ and PfGC-Bcas2−/− ovaries. The areas circled by the dotted white lines represent granulosa cells. The white arrow indicates PfGC-Bcas2+/+ granulosa cells, and the yellow arrow indicates PfGC-Bcas2−/− granulosa cells. Scale bars, 50 μm