Fig. 4
From: BCAS2 regulates granulosa cell survival by participating in mRNA alternative splicing
Loss of BCAS2 results in decreased BrdU+cells in granulosa cells. A The expression of PCNA in 10 dpp ovaries by immunofluorescence. Scale bars, 50 μm. B The percentage of PCNA+ in granulosa cells. Student’s t test was used for this study. Data are expressed as the means ± SEMs in at least three independent experiments. * P < 0.05, ** P < 0. 01, *** P < 0.001. C The expression of Ki67 in 10 dpp ovaries by immunofluorescence. Scale bars, 50 μm. D The percentage of Ki67+ granulosa cells. Student’s t test was used for this study. Data are expressed as the means ± SEMs in at least three independent experiments. * P < 0.05, ** P < 0. 01, *** P < 0.001. E Immunofluorescence staining of BrdU in the ovaries of the PfGC-Bcas2+/+ and PfGC-Bcas2−/− mice at 7 dpp, 10 dpp, and 14 dpp. Scale bars, 50 μm. F The percentage of BrdU+ granulosa cells of the PfGC-Bcas2+/+ and PfGC-Bcas2−/− mice at 7 dpp, 10 dpp, and 14 dpp. Student’s t test was used for this study. Data are expressed as the means ± SEMs in at least three independent experiments. * P < 0.05, ** P < 0. 01, *** P < 0.001