Fig. 6
From: BCAS2 regulates granulosa cell survival by participating in mRNA alternative splicing
Loss of BCAS2 in granulosa cells leads to changes in cell cycle, apoptosis-related mRNA. A Volcano map of differentially expressed transcripts in 8 dpp PfGC-Bcas2+/+ and PfGC-Bcas2−/− ovaries. Green dots are downregulated transcripts, red dots are upregulated transcripts, and blue dots are unchanged transcripts (pvalue ≤ 0.05, |log2FoldChange|≥0). B Cluster heatmap. Hierarchical clustering was used to cluster the FPKM values of genes, and rows were homogenized (Z score). C KEGG dot analysis of the significantly affected transcripts. D Real-time PCR analysis of the differentially expressed genes. Student’s t test was used for this study. Data are expressed as the means ± SEMs in at least three independent experiments. * P < 0.05, ** P < 0. 01