Fig. 7
From: BCAS2 regulates granulosa cell survival by participating in mRNA alternative splicing
BCAS2 participates in AS by forming the PRP19 complex. A Five AS events significantly affected by BCAS2-deficient granulosa cells at 8 dpp. rMATS (3.2.5) software was used to analyse the AS (PDR < 0.5). B Pie chart was used to show the proportion of different AS types. C GO enrichment analysis scatter plot from the 163 affected AS events. D IGV visualization software shows E2f3 and Flt3l AS. E RT‒PCR verification of AS in PfGC-Bcas2+/+ and PfGC-Bcas2−/−. Primers were synthesized by Sangon Biotech (Supplementary Table 4). Arrowheads indicate abnormal AS. F Western blot of E2F3 and FLT3L expression at 8 dpp in the PfGC-Bcas2+/+ and PfGC-Bcas2−/− ovaries. G Co-IP analysis of BCAS2 interacts with PRP19 and CDC5L